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Computationally designed hyperactive Cas9 enzymes.
Vos PD, Rossetti G, Mantegna JL, Siira SJ, Gandadireja AP, Bruce M, Raven SA, Khersonsky O, Fleishman SJ, Filipovska A, Rackham O. Vos PD, et al. Nat Commun. 2022 May 31;13(1):3023. doi: 10.1038/s41467-022-30598-9. Nat Commun. 2022. PMID: 35641498 Free PMC article.
Mutational rescue of the activity of high-fidelity Cas9 enzymes.
Vos PD, Gandadireja AP, Rossetti G, Siira SJ, Mantegna JL, Filipovska A, Rackham O. Vos PD, et al. Cell Rep Methods. 2024 Apr 22;4(4):100756. doi: 10.1016/j.crmeth.2024.100756. Epub 2024 Apr 11. Cell Rep Methods. 2024. PMID: 38608689 Free PMC article.